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Main article: Preimplantation genetic diagnosis. Further information: Final maturation induction. ICSI is also used when sperm numbers are very low. Theoretically, IVF could be performed by collecting the contents from a woman's fallopian tubes or uterus after natural ovulation, mixing it with sperm , and reinserting the fertilised ova into the uterus. Centers for Disease Control and Prevention. A Victorian federal court had ruled in that the existing ban on all single women and lesbians using IVF constituted sex discrimination. In addition, PGS can significantly reduce the risk of multiple pregnancies because fewer embryos, ideally just one, are needed for implantation. If semen is being provided by a sperm donor , it will usually have been prepared for treatment before being frozen and quarantined, and it will be thawed ready for use.

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